A pan-genome method to determine core regions of the Bacillus subtilis and Escherichia coli genomes [version 1; peer review: 2 approved]
Background: Synthetic engineering of bacteria to produce industrial products is a burgeoning Yanagiba Knives field of research and application.In order to optimize genome design, designers need to understand which genes are essential, which are optimal for growth, and locations in the genome that will be tolerated by the organism when inserting eng